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Product Info Summary
| SKU: | M32657 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-beta Tubulin
SKU/Catalog Number
M32657
Size
50 µl
Description
Boster Bio Anti-beta Tubulin (Catalog # M32657). Tested in WB, Flow Cytometry, IF application(s). This antibody reacts with Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-beta Tubulin (Boster Biological Technology, Pleasanton CA, USA, Catalog # M32657)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 46-78 amino acids from human.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M32657 is reactive to Tubb5 in Mouse, Rat
Applications
M32657 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
49.671kDa
Background of Tubb5
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:2000
FC: 1:25
Validation Images & Assay Conditions
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C2C12 (mouse myoblast cell line) cells labeling beta Tubulin with M32657 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on C2C12 cell line. The nuclear counter stain is DAPI (blue).
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All lanes : Anti-beta Tubulin at 1:2000 dilution
Lane 1: C2C12 whole cell lysate
Lane 2: L6 whole cell lysate
Lane 3: mouse spleen lysate
Lane 4: mouse thymus lysate
Lane 5: NIH/3T3 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 50 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing NIH/3T3 cells stained with M32657 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M32657, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Protein Target Info & Infographic
Gene/Protein Information For Tubb5 (Source: Uniprot.org, NCBI)
Gene Name
Tubb5
Full Name
Tubulin beta-5 chain
Weight
49.671kDa
Superfamily
tubulin family
Alternative Names
Tubulin beta-5 chain Tubb5|AA408537, AI596182, B130022C14Rik, M(beta)5|tubulin, beta 5 class I|tubulin beta-5 chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Tubb5, check out the Tubb5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Tubb5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-beta Tubulin (M32657)
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No publications found for M32657
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Customer Reviews
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